In order to explore the effects of organic fertilizer on dry matter, nutrients accumulation and distribution in Pueraria thomsonii Benth, three treatments were included: single application of chemical fertilizer (CK), single application of organic fertilizer (OM) and 50 % chemical fertilizer + 50 % organic fertilizer (CM). The changes of dry matter, nutrient accumulation and distribution ratio in different organs of Pueraria thomsonii Benth at different growth stages were analyzed. The results showed that, the total accumulation of dry matter and nutrients in OM and CM treatments was significantly lower than CK at elongation and expansion stages except for P during elongation. There was no significant difference in dry matter and nutrient accumulation among different treatments at harvest stage. Compared with CK, treatments containing organic fertilizers (OM, CM) significantly reduced nutrient accumulation in branches and leaves at harvest (P < 0.05) , but increased the dry matter distribution ratio of root tubers at harvest (8.53% to 12.5%). The treatments with organic fertilizer can continuously supply nutrients for Pueraria thomsonii benth at the middle and late growth stages, improve the nutrient accumulation of root tubers, and thereby improve root tuber yield of Pueraria lobata root, especially the CM treatment.