Abstract: As one of the main sources of agricultural non-point source pollution, livestock manure poses a serious threat to the quality of farmland soil and water quality in the river source area.In this study, the livestock manure in Dongliao County, the source area of the Dongliao River was selected to analyze its heavy metals and typical antibiotics and their production characteristics, and the environmental carrying capacity of livestock manure farmland application and countermeasures were clarified.The results showed that the heavy metals Cu and Zn contents in livestock manure and chlortetracycline residues in tetracycline antibiotics were with the highest values, and the relevant contents of chlortetracycline residues in pig manure were higher than those in chicken manure and cattle manure.Assuming that all the livestock and poultry manure produced in Dongliao County were returned to the field in the same year, based on the heavy metal background values, the single factor pollution index was greater than 1 level and less than 2 level, reaching the light pollution level.While the heavy metal comprehensive pollution index was 0.97, reaching the warning pollution levels.When the safe application period of livestock manure was 100 years, the minimum application radius of dry land and paddy field were 233 m and 44.5 m, respectively, and the ratio to the maximum application radius was 0.25.The ecological risk values of antibiotics were all less than 0.01, and there was no ecological risk.Therefore, the heavy metals Cu, Zn, and Hg should be treated in a harmless manner before the livestock manure is applied to farmland.