Research Progress of Bacteriophages Infecting Ralstonia Solanacearum
-
摘要: 烟草等茄科植物青枯病的防治是世界性难题,传统的化学方法、轮作及种植抗病品种等都不能有效控制该病害。探索一种新型、有效的生物防治方法——"噬菌体疗法"成为近年来研究的热点。噬菌体应用于细菌性疾病或病害的防治可以追溯到上世纪初,然而伴随着抗生素的发现和广泛应用,噬菌体疗法在相当长的时间里被忽略。近年来,随着耐药性细菌和超级细菌的出现,利用噬菌体防控细菌性疾病和病害的研究越来越受到人们的重视。本文简单阐述了噬菌体的早期研究与复苏的历史,青枯雷尔氏菌噬菌体的获得方法、分类及潜在应用,指出了噬菌体应用研究中存在的问题,并对其以后的发展进行了展望。Abstract: Bacterial wilt is a destructive disease caused by Ralstonia solanacearum and it is distributed in many countries of the world. This disease commonly occurrs in many plants, such as tobacco, eggplant and tomato, etc. Traditional methods, such as using chemicals, crop rotation, planting disease-resistant variety and regulating seedling transportation are not fully effective in controlling this plant disease. Therefore, to exploit an alternative newly and highly effective control method, the phage therapy was highlighted in recent years. Historically, the study of using bacteriophages to control bacterial diseases in human and plants can be dated back to the beginning of the last century;however, with the development and wide application of antibiotics, the phage therapy was neglected for a long times. Recently, with the appearance of drug-resistant bacteria and superbacteria, the study of using phage therapy to control bacterial diseases has been paid much attention. In this paper, we briefly reviewed the initial and revival research history of phages, the method of isolation, taxonomy and potential application of phages infecting Ralstonia solanacearum. Finally, several problems and future research tendencies of phage therapy were also pointed out.
-
Keywords:
- Ralstonia solanacearum /
- bacteriophage /
- phage therapy /
- biocontrol
-
-
[1] Hayward A C.Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum[J]. Annual Review of Phytopathology, 1991, 29(1):65-87.
[2] Lucas G B.Diseases of tobacco[M].Raleigh, North, Carol, USA:Biological Consulting Associates, 1975.
[3] 霍沁建, 张深, 王若焱.烟草青枯病研究进展[J]. 中国农学通报, 2007, 23(8):364-368. [4] 郭坚华, 孙平华, 吴云波, 等.植物细菌性青枯病的生物防治机制和途径[J]. 中国生物防治学报, 1997, 13(1):42-46. [5] 张竹青, 罗宽, 高必达, 等.七株抗青枯病菌生防菌的初步鉴定[J]. 湖南农业大学学报(自然科学版), 2002, 28(6):512-513. [6] 卢洪兴, 曾军, 邱志丹, 等.烟草青枯病发生与药剂防治研究[J]. 福建农业学报, 1996, 11(3):41-45. [7] 纪成灿, 方树民, 顾钢, 等.烟草品种抗青枯病鉴定中的相关因素分析[J]. 中国烟草科学, 2000, 21(2):1-4. [8] 蔡刘体, 汪汉成, 袁赛飞, 等.青枯菌特异性噬菌体的研究进展与应用[J]. 生物技术通讯, 2012, 23(6):887-890. [9] D'Herelle F.Surun microbe invisible antagoniste des bacilles dysentériques[J]. Comptes Rendus de L'Acadernie des Sciences de Paris, 1917, 165:373-375.
[10] Twort F W.An investigation on the nature of ultra-microscopic viruses[J]. The Lancet, 1915, 186(4814):1241-1243.
[11] 王盛, 童贻刚.噬菌体治疗研究进展[J]. 微生物学通报, 200936(7):1019-1024. [12] Bruynoghe R, Maisin J.Essais de thérapeutique au moyen du bacteriophage[J]. Comptes rendus hebdomadaires de Seances de la Societe de Biologie, 1921, 85:1120-1121.
[13] Okabe N, Goto M.Bacteriophages of plant pathogens[J]. Annual Review of Phytopathology, 1963, 1(1):397-418.
[14] Goto M.Fundamentals of bacterial plant pathology[M].New York:Academic Press, 1992.
[15] Elizabeth K, Alexander S.Bacteriophages:Biology and Applications[M].Boca Raton, Florida:Taylor and Francis Group Press, 2005.
[16] 赵勇.噬菌体治疗研究进展[J]. 畜牧兽医科技信息, 2002, 18(4):14-16. [17] Slopek S, Weber-Dabrowska B, Dabrowski M, et al.Results of bacteriophage treatment of suppurative bacterial infections in the years 1981-1986[J]. Archivum Immunologiae Et Therapiae Experimentalis, 1987, 35(5):569-583.
[18] Kutter E.Phage therapy:Bacteriophages as antibiotics[J]. http://www.evergreen.edu/phage/phagetherapy/phagetherapy.html,1997.
[19] Solodovnikov L, Pavlova L I, Emel'ianov P I, et al.The prophylactic use of dry polyvalent dysentery bacteriopathe with pectin in preschool children's institutions.I.Results of a strictly controlled epidemiologic trial(Yaroslavl, 1968)[J]. Zhurnal Mikrobiologii, Epidemiologii, I Immunobiologii, 1970, 47(5):131-137.
[20] 陈晓春, 王继文, 曹永长, 等.噬菌体在疾病治疗方面的应用及研究[J]. 动物医学进展, 2005, 26(1):32-35. [21] 成伏波, 郑文旭, 李菁华, 等.噬菌体疗法对烧伤合并铜绿假单胞菌感染小鼠的治疗作用[J]. 中国感染与化疗杂志, 2009, 9(1):18-21. [22] 张立丽, 李宁, 赵秀英.噬菌体治疗研究进展[J]. 北京医学, 2011, 33(3):232-235. [23] Osawa S, Furuse K, Watanabe I.Distribution of ribonucleic acid coliphages in animals[J]. Applied and Environmental Microbiology, 1981, 41(1):164-168.
[24] Greer G G.Bacteriophage control of foodborne bacteria[J]. Journal of Food Protection, 2005, 68(5):1102-1111.
[25] Leverentz B, Conway W S, Alavidze Z, et al.Examination of bacteriophage as a biocontrol method for Salmonella on fresh-cut fruit:A model study[J]. Journal of Food Protection, 2001, 64(8):1116-1121.
[26] Leverentz B, Conway W S, Camp M J, et al.Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin[J]. Applied and Environmental Microbiology, 2003, 69(8):4519-4526.
[27] Carlton R M.Phage therapy:past history and future prospects[J]. Archivum Immunologiae et Therapiae Experimentalis, 1999, 47(5):267-274.
[28] Zaccardelli M, Saccardi A, Gambin E, et al.Xanthomonas campestris pv.pruni bacteriophages on peach trees and their potential use for biological control[J]. Phytopathologia Mediterranea, 1992, 31(3)133-140.
[29] Balogh B, Jones J B, Momol M T, et al.Persistence of bacteriophages as biocontrol agents in the tomato canopy[J]. Acta Horticulturae, 2005, 695(695):299-302.
[30] 冯烨, 刘军, 孙洋, 等.噬菌体最新分类与命名[J]. 中国兽医学报, 2013, 33(12):1954-1958. [31] 高苗, 杨金广, 刘旭, 等.一株裂解性青枯雷尔氏菌噬菌体的分离及生物学特性分析[J]. 中国农业科学, 2015, 48(7):1330-1338. [32] Van T T B, Yoshida S, Miki K, et al.Genomic characterization of φRS603, a filamentous bacteriophage that is infectious to the phytopathogen Ralstonia solanacearum[J]. Microbiology and Immunology, 2014, 58(12):697-700.
[33] Thi B V T, Khanh N H P, Namikawa R, et al.Genomic characterization of Ralstonia solanacearum phage φRS138 of the family Siphoviridae[J]. Archives of Virology, 2016, 161(2):483-486.
[34] Van T T B, Yoshida S, Miki K, et al.Complete genome sequence of a filamentous bacteriophage, RS611, that infects the phytopathogen Ralstonia solanacearum[J]. Archives of Virology, 2015, 160(4):1143.
[35] Fujiwara A, Kawasaki T, Usami S, et al.Genomic characterization of Ralstonia solanacearum phage φRSA1 and its related prophage (φRSX)in strain GMI1000[J]. Journal of Bacteriology, 2008, 190(1):143-156.
[36] Kawasaki T, Shimizu M, Satsuma H, et al.Genomic characterization of Ralstonia solanacearum phage φRSB1, a T7-like wide-host-range phage[J]. Journal of Bacteriology, 2009, 191(1):422-427.
[37] Bhunchoth A, Phironrit N, Leksomboon C, et al.Isolation of Ralstonia solanacearum-infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents[J]. Journal of Applied Microbiology, 2015, 118(4):1023-1033.
[38] Bhunchoth A, Blanc-Mathieu R, Mihara T, et al.Two asian jumbo phages, φRSL2 and φRSF1, infect Ralstonia solanacearum and show common features of φKZ-related phages[J]. Virology, 2016, 494:56-66.
[39] Yamada T, Satoh S, Ishikawa H, et al.A jumbo phage infecting the phytopathogen Ralstonia solanacearum defines a new lineage of the Myoviridae family[J]. Virology, 2010, 398(1):135-147.
[40] Kawasaki T, Nagata S, Fujiwara A, et al.Genomic characterization of the filamentous integrative bacteriophages φRSS1 and φRSM1, which infect Ralstonia solanacearum[J]. Journal of Bacteriology, 2007, 189(16):5792-5802.
[41] Askora A, Kawasaki T, Usami S, et al.Host recognition and integration of filamentous phage φRSM in the phytopathogen, Ralstonia solanacearum[J]. Virology, 2009, 384(1):69-76.
[42] Makari Hanumanthappa K, Palaniswamy M, Angayarkanni J.Isolation of lytic bacteriophage against Ralstonia solanacearum causing wilting symptoms in ginger(Zingiber officinale) and potato (Solanum tuberosum) plants[J]. International Research Journal of Biological Sciences, 2013, 2(11):78-84.
[43] Salanoubat M, Genin S, Artiguenave F, et al.Genome sequence of the plant pathogen Ralstonia solanacearum[J]. Nature, 2002, 415(6871):497-502.
[44] Kutin R K, Alvarez A, Jenkins D M.Detection of Ralstonia solanacearum in natural substrates using phage amplification integrated with real-time PCR assay[J]. Journal of Microbiological Methods, 2009, 76(3):241-246.
[45] Tanaka H, Negishi H, Maeda H.Control of tobacco bacterial wilt by an avirulent strain of Pseudomonas solanacearum M4S and its bacteriophage[J]. Japanese Journal of Phytopathology, 1990, 56(2):243-246.
[46] Fujiwara A, Fujisawa M, Hamasaki R, et al.Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages[J]. Applied and Environmental Microbiology, 2011, 77(12):4155-4162.
[47] 朱育玮, 李玉保, 王守荣, 等.噬菌体及其治疗细菌感染的研究进展[J]. 中国畜牧兽医, 2015, 42(3):769-773. [48] Gill J, Abedon S T.Bacteriophage ecology and plants[J]. The American Phytopathological Society Features, 2003.
[49] Fegan M, Prior P.How complex is the "Ralstonia solanacearum species complex"[M].In Allen C, Prior P, Hayward A C, edc.Bacterial wilt disease and the Ralstonia solanacearum species complex.Minnesota, USA:American Phytopathological Society Press, 2005.
[50] 赵晨, 王辂.噬菌体治疗——旧概念, 新阶段[J]. 微生物学通报, 2011, 38(11):1698-1704. [51] Payne R J H, Jansen V A A.Phage therapy:The peculiar kinetics of self-replicating pharmaceuticals[J]. Clinical Pharmacology & Therapeutics, 2000, 68(3):225-230.
[52] 高成秀, 严亚贤.噬菌体治疗制剂的研究进展[J]. 中国兽医科学, 2007, 37(7):641-644. [53] Carvalho C M, Gannon B W, Halfhide D E, et al.The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickens[J]. BMC Microbiology, 2010, 10:232.
-
期刊类型引用(2)
1. 林志坚，陈长江，周挺，顾钢，胡方平，李春英，蔡学清. 青枯菌噬菌体RPZH6株系对烟草青枯病的生防效果及全基因组测序分析. 中国农业科技导报. 2022(10): 133-142 . 
百度学术
2. 刘露，林志坚，周挺，梁颁捷，顾钢，胡方平，蔡学清. 植物青枯菌噬菌体保存方法的研究. 植物保护. 2021(04): 155-160 . 百度学术
其他类型引用(11)
计量
- 文章访问数: 128
- HTML全文浏览量: 13
- PDF下载量: 7
- 被引次数: 13
下载:
